Explore T-Cell Bispecific Antibodies

Jump to:
  • Platforms

T-cell bispecifics are an innovative method of harnessing the immune system to fight cancer

Tumours may employ various mechanisms that result in an insufficient number of activated and/or antigen-specific T cells within the tumour microenvironment.1 One approach to redirect and expand populations of T cells is the development of T-cell bispecific antibodies, which can engage any type of T cell, independent of their antigen specificity to cancer cells.2-4

T-cell bispecific antibodies use recombinant antibody–based technology to simultaneously bind to CD3ε, a component of the T-cell receptor (TCR) complex, and to a tumour antigen (TA). Simultaneous binding of a T-cell bispecific antibody to a T cell and a tumour cell results in crosslinking of the TCR, subsequent downstream signalling, and formation of an immunological synapse that results in tumour killing through4

  • T-cell activation and secretion of cytotoxic granules
  • T-cell activation and secretion of cytokines/chemokines that generate a pro-inflammatory tumour microenvironment and recruit additional T cells from the circulation
  • T-cell proliferation and expansion at the tumour site

T-cell bispecific bond

T-cell bispecific antibody–mediated tumour-cell killing does not require antigen presentation and is independent of T-cell specificity, activation, and differentiation status.4,5 Therefore, T-cell bispecific antibodies may be used to promote antitumour immune activity while bypassing steps in the cancer immunity cycle.4,6,7


Roche is exploring the potential of 2 innovative T-cell bispecific antibody platforms in reinitiating antitumour immune responses across various tumour types



The 2:1 T-Cell Bispecific (TCB) platform represents a novel approach to reviving T-cell–mediated immune responses in a variety of tumours

Roche’s 2:1 TCB technology facilitates bivalent binding to a tumour antigen (TA) and monovalent binding to the CD3ε subunit of the T-cell receptor (TCR) complex.4 The 2:1 TCB antibody is equipped with structural features that promote tumour targeting and retention, resulting in a robust, antitumour effect.7

T-cell bispecific


The 1:1 T-cell Dependent Bispecific (TDB) platform utilises native antibody architecture to revive T-cell–mediated immune responses in a variety of tumours

Roche’s 1:1 TDB technology facilitates simultaneous binding to a tumour antigen (TA) and to CD3ɛ on T cells. The 1:1 TDB antibody is equipped with structural features that promote T-cell recruitment and retention, resulting in a robust, antitumour effect.8,9

T-cell Dependent Bispecific

Back to top
  1. Chen DS, Mellman I. Elements of cancer immunity and the cancer-immune set point. Nature. 2017;541:321-330. PMID: 28102259
  2. Kontermann RE, Brinkmann U. Bispecific antibodies. Drug Discov Today. 2015;20:838-847. PMID: 25728220
  3. Frankel SR, Baeuerle PA. Targeting T cells to tumour cells using bispecific antibodies. Curr Opin Chem Biol. 2013;17:385-392. PMID: 23623807
  4. Bacac M, Klein C, Umana P. CEA TCB: a novel head-to-tail 2:1 T cell bispecific antibody for treatment of CEA-positive solid tumours. Oncoimmunology. 2016;5:e1203498. PMID: 27622073
  5. Davis MM. A new trigger for T cells. Cell. 2002;110:285-287. PMID: 12176315
  6. Chen DS, Mellman I. Oncology meets immunology: the cancer-immunity cycle. Immunity. 2013;39:1-10. PMID: 23890059
  7. Bacac M, Fauti T, Sam J, et al. A novel carcinoembryonic antigen T-cell bispecific antibody (CEA TCB) for the treatment of solid tumours. Clin Cancer Res. 2016;22:3286-3297. PMID: 26861458
  8. Sun LL, Ellerman D, Mathieu M, et al.  Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies. Sci Transl Med. 2015;7:1-10. PMID: 25972002
  9. Sun LL, Wang P, Clark R, et al. Preclinical characterization of combinability and potential synergy of anti-CD20/CD3 T-cell dependent bispecific antibody with chemotherapy and PD-1/PD-L1 blockade. Blood. 2016;128:4168.

For offline use follow these steps

By accessing this website from your mobile or tablet, you will be able to download its content for offline use.

  • iOS
  • Android

1. Access the website from your tablet or mobile using Safari as the browser. Make sure you aren’t in a private navigation window. 
2. Click on () on your browser window
3. Select “Add to Home Screen”


1. Access the website from tablet or mobile using Chrome as the browser. Make sure you aren't in a private navigation window. 
2. Click on the menu icon ( ). 
3. Select "Add to homescreen"